Gwen Stacy is portrayed by Bryce Dallas Howard in Spider-Man 3. She is a potential new love interest for Peter Parker, serving as a rival to Mary Jane Watson. Gwen is a classmate and lab partner of Peter, who (as Spider-Man) rescues her early in the film from a construction crane accident. She kisses an upside-down Spider-Man (the same way MJ kissed Spider-Man in the rain in the first film) which causes MJ to become angry and hurt. As Peter is at the top of Dr. Connors's quantum mechanics class, he tutors her; Peter told MJ science was not Gwen's strongest subject. She considers Peter a genius and is very fond of him.
She is also in a relationship with Eddie Brock, who (as a friend) took pictures of her so Gwen could be a model. Eddie mistakes her casual friendship for the same kind of romantic attraction he feels for her. This relationship is short-lived, as Peter Parker, under the influence of the symbiote, steals her from Eddie (fueling his hatred for Parker) and goes out on a date with her. He dances with her at the same jazz club where MJ works, but Gwen realizes that Peter is doing this to make MJ jealous and as if Peter has moved on and does not care about her, she apologizes to Mary Jane, and leaves. After the events at the jazz club, Peter rejects the symbiote and it takes hold of Eddie, transforming him into Venom. Venom then kidnaps Mary Jane and tells Peter, "You made me lose my girl. Now I'm gonna make you lose yours." Gwen is later present at Harry Osborn's funeral.